INCFinder

GenBank ID	LC807806.1
Plasmid name	pD226
Classification	-
Length	109 131 bp
GC content	51%
Organism	Escherichia coli D226
Curation	Manually annotated

Plasmid map

Notes

Large parts of the IncC backbone including RHS, orf1847/1832 genes and the sites of I1 and I2 insertions are missing. The plasmid shows higher homology to pR148 than to pR55, but cannot be classified into Type1/2.

Characteristic genes

Gene name	Length	Location	Length of alignment	Identity (%)	Coverage (%)	E-value
repA	1101	84981 - 86081 (-)	1101	100	100	0
AriB	640	6738 - 7377 (-)	640	100	24	0
AriB	386	2384 - 2769 (-)	386	100	15	0

Antimicrobial resistance genes

Type match	Model name	Model type	Location	E-value	Identity
Strict	Erm(42)	protein homolog model	1441 - 2169(+)	1.59676e-173	99.16 %
Erm 23S ribosomal RNA methyltransferase AMR Gene Family Erm proteins are part of the RNA methyltransferase family and methylate A2058 (E. coli nomenclature) of the 23S ribosomal RNA conferring degrees of resistance to Macrolides, Lincosamides and Streptogramin b. This is called the MLSb phenotype. macrolide antibiotic Drug Class Macrolides are a group of drugs (typically antibiotics) that have a large macrocyclic lactone ring of 12-16 carbons to which one or more deoxy sugars, usually cladinose and desosamine, may be attached. Macrolides bind to the 50S-subunit of bacterial ribosomes, inhibiting the synthesis of vital proteins. lincosamide antibiotic Drug Class Lincosamides (e.g. lincomycin, clindamycin) are a class of drugs which bind to the 23s portion of the 50S subunit of bacterial ribosomes. This interaction inhibits early elongation of peptide chains by inhibiting the transpeptidase reaction, acting similarly to macrolides. streptogramin antibiotic Drug Class Streptogramin antibiotics are natural products produced by various members of the Streptomyces genus. These antibiotics bind to the P site of the 50S subunit of bacterial ribosomes to inhibit protein synthesis. The family consists of two subgroups, type A and type B, which are simultaneously produced by the same bacterial species in a ratio of roughly 70:30. antibiotic target alteration Resistance Mechanism Mutational alteration or enzymatic modification of antibiotic target which results in antibiotic resistance.
Perfect	Tet(X4)	protein homolog model	4197 - 5354(-)	0	100 %
tetracycline inactivation enzyme AMR Gene Family Enzymes or other gene products which hydroxylate tetracycline and other tetracycline derivatives. Hydroxylation inactivates tetracycline-like antibiotics, thus conferring resistance to these compounds. tigecycline Antibiotic Tigecycline is an glycylcycline antibiotic. It works by inhibiting action of the prokaryotic 30S ribosome. tetracycline Antibiotic Tetracycline is a broad-spectrum polyketide antibiotic produced by many Streptomyces. It works by inhibiting action of the prokaryotic 30S ribosome. glycylcycline Drug Class Glycylcyclines are a new class of antibiotics derived from tetracycline. These tetracycline analogues are specifically designed to overcome two common mechanisms of tetracycline resistance. Presently, there is only one glycylcycline antibiotic for clinical use: tigecycline. It works by inhibiting action of the prokaryotic 30S ribosome, preventing the binding of aminoacyl-tRNA. tetracycline antibiotic Drug Class These antibiotics are derived from tetracycline, a polyketide antibiotic that inhibits the 30S subunit of bacterial ribosomes. antibiotic inactivation Resistance Mechanism Enzymatic inactivation of antibiotic to confer drug resistance.