INCFinder

GenBank ID	CP042646.1
Plasmid name	pNCYU-21-79-1
Classification	C2
Length	184 695 bp
GC content	52%
Organism	Escherichia coli strain NCYU-21-79
Curation	Manually annotated

Plasmid map

Notes

I1 insertion is interrupted by an ISAeme16 (IS630 family) element. The 3' part of RHS is deleted from the 2173 bp of the gene.

Characteristic genes

Gene name	Length	Location	Length of alignment	Identity (%)	Coverage (%)	E-value
repA	1102	141223 - 142324 (-)	1102	100	100	0
ORF1847	5547	61406 - 66947 (-)	5547	100	100	0
I1	354	119910 - 120263 (-)	354	100	83	0
I1	76	121427 - 121502 (-)	76	100	18	1.96692E-35
I2	462	84552 - 85013 (-)	462	100	100	0
AriB	2085	93757 - 95839 (-)	2085	100	79	0
AriB	644	99482 - 100125 (-)	644	99	24	0
RHS	2172	179832 - 182001 (-)	2172	98	59	0

Antimicrobial resistance genes

Type match	Model name	Model type	Location	E-value	Identity
Strict	aadA2	protein homolog model	173110 - 173889(-)	0	99.61 %
ANT(3'') AMR Gene Family Nucleotidylylation of streptomycin at the hydroxyl group at position 3'' spectinomycin Antibiotic Spectinomycin is an aminoglycoside antibiotic used to treat different types of bacterial infections. Spectinomycin works by binding to the bacterial 30S ribosomal subunit inhibiting translation. streptomycin Antibiotic Streptomycin is an aminoglycoside antibiotic used to treat different types of bacterial infections. Streptomycin works by binding to the bacterial 30S ribosomal subunit, causing misreading of mRNA and leaving the bacterium unable to synthesize proteins vital to its growth. aminoglycoside antibiotic Drug Class Aminoglycosides are a group of antibiotics that are mostly effective against Gram-negative bacteria. These molecules consist of aminated sugars attached to a dibasic cyclitol. Aminoglycosides work by binding to the bacterial 30S ribosomal subunit (some work by binding to the 50S subunit), inhibiting the translocation of the peptidyl-tRNA from the A-site to the P-site and also causing misreading of mRNA, leaving the bacterium unable to synthesize proteins vital to its growth. antibiotic inactivation Resistance Mechanism Enzymatic inactivation of antibiotic to confer drug resistance.
Strict	dfrA12	protein homolog model	174309 - 174806(-)	4.60707e-121	99.39 %
trimethoprim resistant dihydrofolate reductase dfr AMR Gene Family Alternative dihydropteroate synthase dfr present on plasmids produces alternate proteins that are less sensitive to trimethoprim from inhibiting its role in folate synthesis, thus conferring trimethoprim resistance. trimethoprim Antibiotic Trimethoprim is a synthetic 5-(3,4,5- trimethoxybenzyl) pyrimidine inhibitor of dihydrofolate reductase, inhibiting synthesis of tetrahydrofolic acid. Tetrahydrofolic acid is an essential precursor in the de novo synthesis of the DNA nucleotide thymidine. Trimethoprim is a bacteriostatic antibiotic mainly used in the prophylaxis and treatment of urinary tract infections in combination with sulfamethoxazole, a sulfonamide antibiotic. diaminopyrimidine antibiotic Drug Class Diaminopyrimidines are a class of organic compounds containing a pyrimidine ring substituted by two amine groups. They are inhibitors of dihydrofolate reductase, an enzyme critical for DNA synthesis. antibiotic target replacement Resistance Mechanism Replacement or substitution of antibiotic action target, which process will result in antibiotic resistance.